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KMID : 0364820170530040292
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Korean Journal of Microbiology
2017 Volume.53 No. 4 p.292 ~ p.296
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Effect of Tex1/THOC3, a component of THO complex, on growth and mRNA export in fission yeast
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Bae Soo-Jeong
Koh Eun-Jin
Yoon Jin-Ho
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Abstract
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In eukaryote, THO/TREX complex plays a critical role in transcriptional elongation, pre-mRNA processing, and nuclear mRNA export. This complex is evolutionally well- conserved, but there are some differences in composition and function according to organisms. Here we showed that spTex1, a component of THO/TREX complex, is not essential for growth and mRNA export in a fission yeast, Schizosaccharomyces pombe, which is more similar to higher eukaryote than budding yeast. Deletion and overexpression of the spTex1 gene do not lead to any detectable growth phenotype and accumulation of poly(A)+ RNA in the nucleus. And the spTex1-GFP protein is localized mainly in the nucleus. Yeast two-hybrid and Coimmunoprecipitation analysis showed that the spTex1 protein interacted with spHpr1 (THOC1) and spTho2 (THOC2), main subunits of THO complex. We conclude that the S. pombe Tex1 is a component of THO/TREX complex, but does not plays important roles in growth and bulk mRNA export from the nucleus.
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KEYWORD
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Schizosaccharomyces pombe, mRNA export, Tex1/ THOC3, THO/TREX complex
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